Assessment of Invitro Antibacterial Activity of Moringa oléifera and Murraya koenigii Leaf Extracts Against Clinically Important Bacteria

Resistant gram-negative bacteria are increasing in prevalence, causing health concerns, particularly in hospitals and intensive care units, leading to increased healthcare expenses due to sickness, and death. One frequent bacterial infection that affects many people is Urinary Tract Infection (UTI). Objective: This study was conducted to assess the anti-microbial activity of leaf extracts from Murraya Koenigii (Mk) and Moringa oleifera (Mo) against multidrug-resistant Klebsiella pneumoniae (MDR-Kp) in vitro. Methods: It was a Preclinical in-vitro study, carried out at Ziauddin University from December 2022 to May 2023. Using a rotary evaporator, MO and MK leaves were extracted. Utilizing the Agar well diffusion assay and the broth dilution assay, the antibacterial activity of both plants were assessed. Results: For both extracts, concentrations ranging from 7.812 mg/ml to 500 mg/ml were prepared in 10% Dimethyl Sulfoxide (DMSO). Minimum Inhibitory Concentration (MIC) of Murraya Koenigii leaf extract was found to be 15mg/ml against MDR-Kp. Moringa oleifera leaf extract did not exhibit any discernible antibacterial action against MDR-Kp at any of the tested concentrations. Conclusion: While MOLE did not impede the growth of MDR-Kp strains at the tested doses, MKLE hindered the growth of MDR-Kp strains at 15 mg/ml (MIC).