Conservation of symbiotic signaling since the most recent common ancestor of land plants
Plants have colonized lands 450 million years ago. This terrestrialization was facilitated by developmental and functional innovations. Recent evo-devo approaches have demonstrated that one of these innovations was the mutualistic arbuscular mycorrhizal symbiosis (AMS). The genetic pathways that have been involved in the establishment and functioning of AMS since its evolution remain poorly described. Here, we found that intracellular colonization by AM fungi induces a transcriptional reporter of the common symbiosis pathway, well-described in angiosperms, in the liverwort <i>Marchantia paleacea</i>. Mutants of either of the three main genes of this pathway, <i>SYMRK</i>, <i>CCaMK,</i> and <i>CYCLOPS,</i> disrupt the ability of <i>M. paleacea</i> to associate with AM fungi. Finally, overexpressing gain-of-function CCaMK or CYCLOPS leads to convergent transcriptomic signatures that partially overlap with AMS. Altogether, our data indicate that plants have maintained three genes of the common symbiotic pathway to support symbiotic interactions since their most recent common ancestor.
Authors
- Camille Girou
- Didier Reinhardt (ORCID: https://orcid.org/0000-0003-3495-6783)
- Pierre‐Marc Delaux (ORCID: https://orcid.org/0000-0002-6211-157X)
- Cyril Libourel (ORCID: https://orcid.org/0000-0002-4271-9596)
- Lauréna Medioni (ORCID: https://orcid.org/0000-0003-2315-9818)
- Michiel Vandenbussche (ORCID: https://orcid.org/0000-0002-4192-7369)
- Aurélie Le Ru (ORCID: https://orcid.org/0000-0002-0236-791X)
- Mélanie K. Rich (ORCID: https://orcid.org/0000-0001-6109-1290)
- Jean Keller (ORCID: https://orcid.org/0000-0002-5198-0331)
- P. Morel (ORCID: https://orcid.org/0000-0003-1690-8032)
- Shota Shimazaki (ORCID: https://orcid.org/0000-0001-7557-0479)
- Malick Mbengué (ORCID: https://orcid.org/0000-0002-7723-7757)
- Kyoichi Kodama (ORCID: https://orcid.org/0000-0001-6988-1772)
- Tatiana Vernié (ORCID: https://orcid.org/0000-0003-1387-6370)
- Fabian van Beveren (ORCID: https://orcid.org/0000-0003-2883-3090)
- Junko Kyozuka (ORCID: https://orcid.org/0000-0003-2242-7171)
- Lucie Chauderon
- Eve Teyssier
- Tifenn Pellen
- Vincent Garrigues
- Corinne Lefort
- Yves Martinez (ORCID: https://orcid.org/0000-0002-4132-3813)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- École Normale Supérieure de Lyon (FR)
- Centre National de la Recherche Scientifique (FR)
- Université Toulouse III - Paul Sabatier (FR)
- University of Fribourg (CH)
- Université Fédérale de Toulouse Midi-Pyrénées (FR)
- Tohoku University (JP)
- Laboratoire de Recherche en Sciences Végétales (FR)
- Laboratoire Reproduction et Développement des Plantes (FR)
Publication Details
- Journal
- Proceedings of the National Academy of Sciences
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1073/pnas.2408539121
- Citations
- 20
- Primary Topic
- Mycorrhizal Fungi and Plant Interactions
- Type
- article
- Field-Weighted Citation Impact
- 11.99
Funders
- Agence Nationale de la Recherche
- European Research Council