Evaluation of Wound Healing Potential of Zizyphus jujuba in Rats

The healing potential of a topically applied extract derived from the bark of Ziziphus jujuba was assessed using both incision and excision wound models in albino rats over 20 days. For this study, the rats were categorized into four distinct groups, each group receiving ongoing treatment with 0.5%, 1%, and methanolic extract of Ziziphus jujuba, applied at a dosage of mg/cm2. The test duration encompassed 20 days, during which the diameter of the wounds was measured on days 0, 8, 12, 16, and 20th. Additionally, biopsies were collected from two rats within each group. The findings of the study highlighted that the administration of the lower-dose group of methanolic extract of Ziziphus jujuba (E1 0.5% w/w) was associated with a significant (p lower than 0.01) reduction in wound area. Moreover, the histopathological assessment also corroborated this observation. Notably, during the second and third weeks of the study, the tissues exhibited significant (p lower than 0.01) improvement in comparison to most other groups. Interestingly, the group treated with a higher dose of the methanolic extract of Ziziphus jujuba (E2 1% w/w) displayed the most substantial wound shrinkage and organization. This suggests that the application of the higher extract concentration led to more pronounced wound contraction and enhanced tissue organization.