A Mitochondria‐Targeted Iridium(iii) Phosphorescent Probe for Selective Detection of Hypochlorite in Living Cells
Hypochlorous acid(HClO)/hypochlorite ion (ClO<sup>-</sup>) is a highly reactive oxygen species (ROS) that play a crucial role in various biological processes. In this paper, a "turn-on" phosphorescent probe (Ir-TPP) for detecting ClO<sup>-</sup> in mitochondria was designed and synthesized. In solution, Ir-TPP is minimal emission due to rapid isomerization of C=N-OH as an efficient non-radiative decay process. When Ir-TPP was reacted with ClO<sup>-</sup> to form an oxidized carboxylate product, there is a significant enhancement both in absorbance and emission intensity, resulting in approximately 25-fold increase in phosphorescence signal. Furthermore, the probe demonstrates high selectivity towards other anions and ROS. It also exhibits specific targeting ability towards mitochondria with a co-staining coefficient of 94 % compared to commercial dyes, enabling "turn-on" detection of ClO<sup>-</sup> within mitochondria.
Authors
- Haoxuan Wang (ORCID: https://orcid.org/0000-0002-8663-3073)
- Zhiqiang Pan (ORCID: https://orcid.org/0000-0003-0771-8261)
- Jing Zhang (ORCID: https://orcid.org/0000-0002-1328-719X)
- Tingchun Ma
- Kai Chen
- Wenjuan Xu
- Ying Guang
Institutions
- Bengbu Medical College (CN)
- Nanjing University of Posts and Telecommunications (CN)
- The Synergetic Innovation Center for Advanced Materials (CN)
Publication Details
- Journal
- Chemistry - An Asian Journal
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1002/asia.202401351
- Citations
- 2
- Primary Topic
- Molecular Sensors and Ion Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.48
Funders
- National Natural Science Foundation of China
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Jiangsu National Synergistic Innovation Center for Advanced Materials