First identification and whole genome characterization of rotavirus C in pigs in Zambia
Rotavirus C (RVC) causes acute gastroenteritis in neonatal piglets. Despite the clinical importance of RVC infection, the distribution and prevalence in pig populations in most African countries remains unknown. In this study, we identified RVC in Zambian pigs by metagenomic analysis. The full genome sequence of the RVC revealed two different VP4 sequences, implying that two different RVC strains (ZP18-77-c1 and ZP18-77-c2) were present in the same sample. Genetic analyses demonstrated that all segments of ZP18-77-c1 and ZP18-77-c2 showed high nucleotide sequence identities (87.7-94.5%) to known porcine RVC strains, and ZP18-77-c1 and ZP18-77-c2 strains were assigned to genotype constellations, G1-P[4]/P[14]-I13-R5-C5-M1-A7-N9-T10-E5-H1. We further screened RVC genomes among pig feces collected in Zambia (n = 147) by RT-qPCR, and 78 samples (53.1%) were positive. This study demonstrated the first full genome sequence of African RVC strains with a relatively high prevalence of RVC infection in the pig populations in Zambia.
Authors
- Keita Matsuno (ORCID: https://orcid.org/0000-0002-4205-6526)
- Joseph Ndebe (ORCID: https://orcid.org/0000-0002-3070-8830)
- Hirofumi Sawa (ORCID: https://orcid.org/0000-0003-2569-2755)
- Ayato Takada (ORCID: https://orcid.org/0000-0003-2464-6642)
- Masahiro Kajihara
- Yasuko Orba (ORCID: https://orcid.org/0000-0001-9910-3912)
- Naganori Nao
- Kanako Ishihara (ORCID: https://orcid.org/0000-0001-5881-6078)
- Yongjin Qiu (ORCID: https://orcid.org/0000-0001-8621-5472)
- Michihito Sasaki (ORCID: https://orcid.org/0000-0003-1607-2175)
- Edgar Simulundu (ORCID: https://orcid.org/0000-0001-9423-0816)
- William W. Hall (ORCID: https://orcid.org/0000-0001-7560-3808)
- Aiko Ohnuma
- Bernard M. Hang’ombe (ORCID: https://orcid.org/0000-0002-4201-6401)
- Hayato Harima (ORCID: https://orcid.org/0000-0002-6704-4601)
- Kapila Penjaninge
Institutions
- Hokkaido University of Science (JP)
- Hokkaido University (JP)
- Sapporo International University (JP)
- University of Zambia (ZM)
- Department of Disease Control (TH)
- Golden Valley Agricultural Research Trust (ZM)
- Tokyo University of Agriculture and Technology (JP)
Publication Details
- Journal
- Virology
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1016/j.virol.2024.110385
- Citations
- 1
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.39
Funders
- Japan Agency for Medical Research and Development
- Japan International Cooperation Agency
- Science and Technology Research Partnership for Sustainable Development