Influence of a new combined production based on dense carrot root extract and quercetin on the morphological and proliferative properties of l929 line fibroblasts in cell culture

At the first stage of studying the pharmacological properties of substances, the general cytotoxic effect is determined based on the assessment of viability, morphological integrity and functional activity of cells. One of the standard cell lines often used in cytotoxicity tests is the L929 line. Studying the ability of a combined lipid-lowering agent based on dense carrot root extract and quercetin to influence the morphological and functional properties of fibroblasts of the L929 line in cell culture was the aim of this study. Materials and methods: Determination of the cytotoxicity of the drug under study was carried out in a cell culture of the L929 line at a concentration of 40, 100, and 200 mg/ml. The safety of cells in the resulting suspension was assessed using staining with a 0,4% trypan blue solution. Cell morphology and nuclear-cytoplasmic ratio were assessed in fixed culture preparations stained with hematoxylin-eosin. To assess the migration and proliferative activity of a monolayer culture, the “scratch” test was used. Results: When L929 cells were incubated in a nutrient medium with the addition of the product, their viability and morphological properties were preserved; images of a monolayer of the culture of these cells under phase-contrast microscopy coincided with the quantitative analysis data. The absence of changes in the nuclear-cytoplasmic ratio was further evidence of the absence of toxic effects of the studied drug in relation to fibroblast culture. According to the experiment results, the migration and proliferative activity of L929 cells under the influence of the drug did not differ significantly from intact cells. Conclusions: The results of the toxicological study showed that the combined product based on dense carrot root extract and quercetin does not have a cytotoxic effect on the L929 cell line culture and can be recommended for further preclinical research.