Guidelines for study the oxidative stress damage and mechanism of on <i>Caenorhabditis elegans</i>

Abstract Oxidative stress, caused by free radicals damaging cellular structures, is associated with various diseases. Effective research on oxidative stress mechanisms and potential therapies requires robust model organisms. Caenorhabditis elegans ( C. elegans ) is an ideal model due to its small size, high transparency, and ease of cultivation. Additionally, C. elegans exhibits human‐like responses under oxidative stress, such as changes in antioxidant enzyme activity, accumulation of oxidative damage, and regulation of antioxidant‐related gene expression. This guideline outlines methods for utilizing C. elegans in oxidative stress research, detailing the construction of oxidative stress models and corresponding detection indicators. This comprehensive approach aims to advance the study of oxidative stress and the evaluation of antioxidants.