STUDYING THE RELATIONSHIP BETWEEN SEMINAL PLASMA ENZYMES AND CHEMICAL ELEMENTS CONCENTRATION WITH THE PHYSICAL SEMEN PROPERTIES OF LOCAL AWASSI RAMS

Semen samples were collected by using ten adult Awassi Rams biweekly via electric ejaculator in one ejaculate/ram. Semen properties, enzyme concentration (AST, ALT, ALP, LDH, ACP), and element concentration (Na, K, Ca, Zn, Cl, P, Mg, and Fe) in semen were determined. The results showed that AST enzyme is significantly correlated (r = -0.671), (r = -0.500), (r = -0.587), (r = 0.587) with pH, individual motility, live and dead sperms. ALT enzyme was significantly correlated (r = -0.578), (r = -0.560), (r = 0.560) with pH, live and dead sperms respectively. A negative relationship was found between ALP and pH, mass and individual motility, live sperm, semen volume and sperm membrane integrity.  While, there was a positive relationship between sperm concentration and dead and abnormal sperm. LDH enzyme positively correlated with, mass and individual motility, semen volume, live and abnormal sperms while negatively correlated with pH, sperm concentration, dead sperm, and sperm membrane integrity. ACP enzyme positively correlated with volume, mass and individual motility, dead sperms, sperm concentration, and sperm membrane integrity. Na and Cl were correlated significantly with semen volume and Cl with mass motility. Zn, Ca, Mg, and Fe were positively and significantly correlated with sperm concentration. On the other hand, Ca correlated significantly with individual motility and live and sperm concentration, while this correlation was significantly negative with dead and abnormal sperm. Therefore, the status of the enzymes and minerals of seminal fluids is considered as important metrics for assessing the quality of Awassi rams semen.