A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice
Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins-aflatoxin B<sub>1</sub>, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B<sub>1</sub> in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.
Authors
- Waraporn Panchan
- Manlika Makornwattana (ORCID: https://orcid.org/0009-0004-1854-8493)
- Oluwatobi Kolawole (ORCID: https://orcid.org/0000-0001-5087-4791)
- Narusorn Doljirapisit
- Thanasat Sooksimuang
- Nitsara Karoonuthaisiri (ORCID: https://orcid.org/0000-0003-0838-8399)
- Saowalak Adunphatcharaphon (ORCID: https://orcid.org/0000-0003-0053-4398)
- Grit Pichayawaytin (ORCID: https://orcid.org/0000-0003-2288-0689)
- Awanwee Petchkongkaew (ORCID: https://orcid.org/0000-0001-5738-2191)
- Rungroj Jintamethasawat (ORCID: https://orcid.org/0000-0002-4222-0808)
- Prae Noppakuadrittidej (ORCID: https://orcid.org/0000-0003-1358-4677)
- Armote Somboonkaew (ORCID: https://orcid.org/0000-0002-8849-8952)
- Anuwut Petdum
- Sudtida Kaew-amdee
- Julie Meneely (ORCID: https://orcid.org/0000-0003-3265-9961)
- Christopher T. Elliott
- Wannee Wasuthep
Institutions
- Thammasat University (TH)
- Queen's University Belfast (GB)
- Thailand Institute of Scientific and Technological Research (TH)
- National Science and Technology Development Agency (TH)
- National Electronics and Computer Technology Center (TH)
- National Center for Genetic Engineering and Biotechnology (TH)
Publication Details
- Journal
- npj Science of Food
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1038/s41538-024-00342-2
- Citations
- 10
- Primary Topic
- Mycotoxins in Agriculture and Food
- Type
- article
- Field-Weighted Citation Impact
- 6.00
Funders
- Thailand Science Research and Innovation