Interlaboratory assays from the fungal PCR Initiative and the Modimucor Study Group to improve qPCR detection of Mucorales DNA in serum: one more step toward standardization
Mucormycosis is a life-threatening mold infection affecting immunosuppressed patients but also other patients with diabetes or trauma. Better survival is linked to shorter delays in diagnosis and treatment initiation. Detection of Mucorales-free DNA in serum or plasma using quantitative PCR allows a prompt diagnosis and earlier treatment. Several techniques and protocols of quantitative Mucorales PCR are used in Europe, and improving performance remains a common objective of laboratories participating in the fungal PCR Initiative Working Group. This study, which combined results from 26 laboratories in Europe, showed that the main parameters underpinning sensitivity are the preanalytical variables (volume of serum used for DNA extraction and DNA template volume), irrespective of the extraction platforms and qPCR assay/platform.
Authors
- Willem J. G. Melchers (ORCID: https://orcid.org/0000-0002-5446-2230)
- Pieter‐Jan Haas (ORCID: https://orcid.org/0000-0002-1127-095X)
- Steffi Rocchi (ORCID: https://orcid.org/0000-0001-9262-1127)
- Jürgen Loeffler
- Rosemary A. Barnes (ORCID: https://orcid.org/0000-0002-0574-8670)
- Émeline Scherer (ORCID: https://orcid.org/0000-0003-2123-3703)
- Martina Lengerová (ORCID: https://orcid.org/0000-0001-8739-9998)
- P. Lewis White (ORCID: https://orcid.org/0000-0003-3056-4205)
- Marjorie Cornu (ORCID: https://orcid.org/0000-0002-2075-9861)
- Laurence Millon (ORCID: https://orcid.org/0000-0001-5939-3409)
- Corné H. W. Klaassen (ORCID: https://orcid.org/0000-0002-3439-0903)
- Stefan Fuchs (ORCID: https://orcid.org/0000-0001-8519-0701)
- Rasmus Krøger Hare (ORCID: https://orcid.org/0000-0002-9796-023X)
- Céline Damiani (ORCID: https://orcid.org/0000-0003-3532-6775)
- Philippe Poirier (ORCID: https://orcid.org/0000-0001-9173-2634)
- Jan Springer
- Mario Cruciani (ORCID: https://orcid.org/0000-0002-1641-7367)
- Michaela Lackner (ORCID: https://orcid.org/0000-0001-9073-2886)
- Xavier Iriart (ORCID: https://orcid.org/0000-0002-0998-8962)
- Alexandre Alanio (ORCID: https://orcid.org/0000-0001-9726-3082)
- Damien Dupont (ORCID: https://orcid.org/0000-0002-6992-1073)
- J. Peter Donnelly (ORCID: https://orcid.org/0000-0002-8521-335X)
- María José Buitrago (ORCID: https://orcid.org/0000-0003-1878-6067)
- Ferry Hagen (ORCID: https://orcid.org/0000-0002-5622-1916)
- Julie Denis (ORCID: https://orcid.org/0009-0009-6294-9338)
- Audrey Guitton
- Birgit Willinger (ORCID: https://orcid.org/0000-0001-7921-5749)
- Cristina Mazzi (ORCID: https://orcid.org/0000-0002-4561-3447)
- Françoise Botterel (ORCID: https://orcid.org/0000-0002-3028-2665)
- Florent Morio (ORCID: https://orcid.org/0000-0002-5859-6105)
- Marie‐Elisabeth Bougnoux (ORCID: https://orcid.org/0000-0003-4721-2740)
- Massimo Cogliati (ORCID: https://orcid.org/0000-0002-6842-4817)
- Rebecca Gorton
- Stéphane Valot
Institutions
- Statens Serum Institut (DK)
- Centre National de la Recherche Scientifique (FR)
- Université Toulouse III - Paul Sabatier (FR)
- Innsbruck Medical University (AT)
- Radboud University Nijmegen (NL)
- Inserm (FR)
- Institut Pasteur (FR)
- Université Fédérale de Toulouse Midi-Pyrénées (FR)
- University of Milan (IT)
- Universität Innsbruck (AT)
- Université Paris Cité (FR)
- Heidelberg University (DE)
- Université de Lille (FR)
- University Hospital of Wales (GB)
- Instituto de Salud Carlos III (ES)
- Erasmus MC (NL)
- University Hospital Heidelberg (DE)
- University Medical Center (US)
- Radboud University Medical Center (NL)
- University Medical Center Utrecht (NL)
- Centre Hospitalier Universitaire de Nantes (FR)
- Centre Hospitalier Universitaire Henri-Mondor (FR)
- Public Health Wales (GB)
- Centre Hospitalier Universitaire de Besançon (FR)
- Hôpital de la Croix-Rousse (FR)
- Centre Hospitalier Universitaire Amiens-Picardie (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Biodiversity Research Institute (US)
- Hospices Civils de Lyon (FR)
- Ospedale Sacro Cuore Don Calabria (IT)
- University Hospital Brno (CZ)
- Centro Nacional de Microbiologia (ES)
- Westerdijk Fungal Biodiversity Institute (NL)
- CHU Dijon Bourgogne (FR)
- Unité de Glycobiologie Structurale et Fonctionnelle (FR)
- Universitätsklinikum Würzburg (DE)
- Centre Hospitalier Universitaire de Clermont-Ferrand (FR)
- Hôpital Saint-Louis (FR)
- Hôpitaux Universitaires de Strasbourg (FR)
- Institut Toulousain des Maladies Infectieuses et Inflammatoires (FR)
- Agents infectieux, résistance et chimiothérapie (FR)
- Université de Picardie Jules Verne (FR)
- Medical University of Vienna (AT)
- Cardiff University (GB)
- Université de Franche-Comté (FR)
- Nantes Université (FR)
Publication Details
- Journal
- Journal of Clinical Microbiology
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1128/jcm.01525-24
- Citations
- 9
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 3.50