Minisatellite Isolation and Minisatellite Molecular Marker Development in Citrus limon (L.) Osbeck

Minisatellites are widespread tandem DNA repeats in the genome with a monomer length of 10 to 100 bp. The high variability of minisatellite loci makes them attractive for the development of molecular markers. Minisatellites are used as markers according to three strategies: marking of digested genomic DNA with minisatellite-based probes; amplification with primers based on the sequences of the minisatellites themselves; amplification with primers designed for borders upstream and downstream of the minisatellite locus. In this study, a microsatellite dataset was obtained from the analysis of the Citrus limon (L.) Osbeck genome using Tandem Repeat Finder (TRF) and GMATA software. The minisatellite loci found were used to develop molecular markers that were tested in GMATA using electronic PCR (e-PCR). The obtained dataset includes sequences of extracted minisatellites and their characteristics (start and end nucleotide positions on the chromosome, length of monomer, number of repetitions and length of array), as well as sequences of developed primers, expected lengths of amplicons, and e-PCR results. The presented dataset can be used for the marking of lemon samples according to any of the three strategies. It provides a useful basis for lemon variety certification, identification of samples, verification of collections, lemon genome mapping, saturation of already created maps, studying of the lemon genome architecture etc.