In Vitro Anthelmintic Activity of Crude Extracts of Melissa Officinalis Against Pheretima Posthuma

ABSTRACT: Aims: A literature review indicated that there isn't a single scientific study on the anthelmintic properties of the aerial part of Melissa officinalis. The study's main aim is to determine the anthelmintic activity of fresh juice extract, methanolic extract, and volatile oil extract of Melissa officinalis using Pheretima Posthuma as a test worms’ method. Method and materials: The concentrations of the fresh juice extract, methanolic extract, and volatile oil extract of Melissa officinalis Linn. are 10 mg/ml, 20 mg/ml, 30 mg/ml, 40 mg/ml, and 50 mg/ml. Every extract was examined to ascertain the worms' paralysis and death times. Both extracts exhibited anthelmintic action in vitro. As a standard, albendazole was utilized, while saline water served as the control. Result: Melissa officinalis extracts were tested for anthelmintic action against Pheretima Posthuma (earthworms). Normal saline and albendazole (20 mg/ml) were taken as the control and standard medicine, respectively, while four different concentrations were used. The worms' paralysis and death times were established. According to the results, the fresh juice extract had the highest level of activity. Anthelmintic action was demonstrated in a dose-dependent manner by fresh juice extract, volatile oil extract and methanolic extract. Findings were similar to those of the common medication, albendazole. Conclusion: The aerial part of Melissa officinalis extract showed anthelmintic activity. The results were dose-dependent for various extracts, and they were almost similar to those of the popular drug albendazole. Further investigations are underway to isolate the active principle responsible for the action.