First Report and Phylogenetic Analysis of Mitochondrial Genomes of Chrysomya villeneuvi and Sarcophaga genuforceps

The mitochondrial genome, highly conserved across species, is crucial for species identification, phylogenetic analysis, and evolutionary research. <i>Chrysomya villeneuvi</i> and <i>Sarcophaga genuforceps</i>, two species with significant forensic value, have been understudied in terms of genetic data. In this study, the complete mitochondrial genomes of <i>C. villeneuvi</i> (15,623 bp) and <i>S. genuforceps</i> (15,729 bp) were sequenced and analyzed. All thirteen protein-coding genes (PCGs) exhibited Ka/Ks ratios below one, indicating purifying selection and supporting their utility as barcoding markers. Phylogenetic analysis and genetic distance calculations based on PCGs showed that <i>C. villeneuvi</i> is closely related to <i>Chrysomya rufifacies</i> and <i>Chrysomya albiceps</i>, and <i>S. genuforceps</i> aligns more closely with <i>Sarcophaga kentejana</i> and <i>Sarcophaga schuetzei</i>. This research is the first to provide mitochondrial genome data for <i>C. villeneuvi</i> and <i>S. genuforceps</i>, expanding the genetic resources available for Calliphoridae and Sarcophagidae and offering a foundation for further forensic and evolutionary studies.