Genetic profiling of K232A mutation of DGAT1 gene in Murrah buffalo

The aim of this study was to characterize and validate the candidate K232A in in Murrah buffalo’s Diacylglycerol O-acyltransferase1 (DGAT1) gene. The DGAT1 gene has been identified as a possible candidate gene for milk fat. EaeI restriction enzyme was used to digest a 413 bp PCR product that covered the DGAT1 gene’s exon 7, intron 8, exon 9, and partial exon 9 regions. This allowed us to screen for the reported mutation. Within our resource population, a monomorphic banding pattern with genotype KK was discovered. Additionally, sequencing was done to evaluate and validate the screening mutation in a specific region’s nucleotide sequence. The outcome suggests that the Murrah buffalo has a substantially conserved sequence.