A System for Simultaneous Optogenetics Stimulation, Calcium Imaging, Electrical Signal Detection, and Electrical Stimulation of In Vitro Neuronal Networks

In vitro neuronal networks are promising models for neural computing. Despite numerous efforts to determine the activities of these neuronal networks, achieving high spatiotemporal resolution in recording and controlling neuronal activities remains challenging. Here, we propose the OptoCaMEA system, which simultaneously records neuronal network activities with a temporal resolution of 50 microseconds and a spatial resolution smaller than 10 micrometres. Additionally, it achieves single-neuron control with a spatial resolution smaller than 10 micrometres. OptoCaMEA offers a more precise method for recording and controlling the dynamics of in vitro neuronal networks, facilitating the development of computational frameworks based on biological neural networks and contributing to the in-depth study of related neurological disease models.

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Publication Details

Published
2024-11-02
DOI
https://doi.org/10.1109/acp/ipoc63121.2024.10809785
Primary Topic
Photoreceptor and optogenetics research
Type
article
Field-Weighted Citation Impact
0.00

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article

A System for Simultaneous Optogenetics Stimulation, Calcium Imaging, Electrical Signal Detection, and Electrical Stimulation of In Vitro Neuronal Networks

Xiaojuan Sun, Longze Sha, Yarong Lin, Kun Xu et al.
Photoreceptor and optogenetics research
article

A System for Simultaneous Optogenetics Stimulation, Calcium Imaging, Electrical Signal Detection, and Electrical Stimulation of In Vitro Neuronal Networks

Xiaojuan Sun, Longze Sha, Yarong Lin, Kun Xu, Yueheng Lan, Lili Gui, Zeying Lu, Yin Deng, Qi Xu, Jie Li, Tao Li, Shiyang Cao
article en

Abstract

In vitro neuronal networks are promising models for neural computing. Despite numerous efforts to determine the activities of these neuronal networks, achieving high spatiotemporal resolution in recording and controlling neuronal activities remains challenging. Here, we propose the OptoCaMEA system, which simultaneously records neuronal network activities with a temporal resolution of 50 microseconds and a spatial resolution smaller than 10 micrometres. Additionally, it achieves single-neuron control with a spatial resolution smaller than 10 micrometres. OptoCaMEA offers a more precise method for recording and controlling the dynamics of in vitro neuronal networks, facilitating the development of computational frameworks based on biological neural networks and contributing to the in-depth study of related neurological disease models.

Beijing University of Posts and Telecommunications (CN), Chinese Academy of Medical Sciences & Peking Union Medical College (CN), State Key Laboratory of Information Photonics and Optical Communications
National Natural Science Foundation of China, Beijing University of Posts and Telecommunications, Fundamental Research Funds for the Central Universities
Openalex Percentile: Top 74%
Photoreceptor and optogenetics research
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