Spectral off-axis phase shifting in transmission optical coherence tomography

Transmission optical coherence tomography (tOCT) is gaining interest for imaging biological samples; however, so far, quantitative phase information has not been directly obtained. This study introduces SOPHAST-OCT, an approach for integrated 2D phase recovery in Off-Axis Full-Field Swept-Source tOCT. It uses phase-shifting to leverage OCT’s inherent phase changes between recorded interferograms. SOPHAST-OCT demonstrates promising phase agreement with the simulated ground truth phase. This approach represents a first step toward quantitative imaging in tOCT. Full Text: PDF References Y. Park, C. Depeursinge, G. Popescu, "Quantitative phase imaging in biomedicine", Nature Photon 12, 578 (2018). CrossRef K. Alm, H. Cirenajwis, L. Gisselsson, A.G. Wingren, B. Janicke, A. Mölder, et al., in Holography, Research and Technologies (IntechOpen, 2011). CrossRef W. Drexler, J. Fujimoto, editors , Optical Coherence Tomography: Technology and Applications, 2nd ed. (Springer Cham, 2015). CrossRef W. Krauze, P. Ossowski, M. Nowakowski, M. Szkulmowski, M. Kujawińska, "Enhanced QPI functionality by combining OCT and ODT methods", Quantitative Phase Imaging VII, 11653, 19 (2021). CrossRef B.C. Wilson, S.L. Jacques, "Optical reflectance and transmittance of tissues: principles and applications", IEEE J. Quantum Electron. 26, 2186 (1990). CrossRef J. van der Horst, A.K. Trull, J. Kalkman, "Deep-tissue label-free quantitative optical tomography", Optica 7, 1682 (2020). CrossRef P. Hosseini, Y. Sung, Y. Choi, N. Lue, Z. Yaqoob, P. So, "Scanning color optical tomography (SCOT)", Opt. Expr. 23, 19752 (2015). CrossRef P. de Groot, "Phase Shifting Interferometry" in Optical Measurement of Surface Topography, edited by R. Leach (Springer, Berlin, Heidelberg, 2011). CrossRef M. Wojtkowski, A. Kowalczyk, R. Leitgeb, A.F. Fercher, "Full range complex spectral optical coherence tomography technique in eye imaging", Opt. Lett. 27, 1415 (2002). CrossRef Q. Zhang, S. Zhong, J. Lin, W. Chen, M. Luo, J. Zhong, Y. Yu, Z. Peng, S. Cheng, "Full-range Fourier-domain optical coherence tomography based on Mach–Zehnder interferometer", Opt. Lasers Engin. 124, 105794 (2020). CrossRef R. Juarez-Salazar, C. Robledo-Sanchez, F. Guerrero-Sanchez, A. Rangel-Huerta, "Generalized phase-shifting algorithm for inhomogeneous phase shift and spatio-temporal fringe visibility variation", Opt. Express 22, 4738 (2014). CrossRef R. Juarez-Salazar, C. Robledo-Sánchez, C. Meneses-Fabian, F. Guerrero-Sánchez, L. M. Arévalo Aguilar, "Generalized phase-shifting interferometry by parameter estimation with the least squares method", Opt. Lasers Engin. 51, 626 (2013). CrossRef M.A. Herráez, D.R. Burton, M.J. Lalor, M.A. Gdeisat, "Fast two-dimensional phase-unwrapping algorithm based on sorting by reliability following a noncontinuous path", Appl. Opt. 41, 7437 (2002). CrossRef Z. Wang, A.C. Bovik, "A universal image quality index", IEEE Signal Proc. Lett. 9, 81 (2002). CrossRef