Generation of a collection of MYB mutant lines via pooled CRISPR-Cas9 in grape
● A sgRNA library of CRISPR-Cas9 targeting grape MYB family was designed and synthesized. ● Several grape mutants were obtained via pooled Agrobacterium -mediated transformation. ● A novel grape mutant exhibited significantly enhanced tolerance to drought stress.
Authors
- Ying‐Qiang Wen (ORCID: https://orcid.org/0000-0001-7503-7260)
- Jae Yong Han (ORCID: https://orcid.org/0000-0003-3413-3277)
- Zhuoshuai Jin
- Liang Zhao (ORCID: https://orcid.org/0000-0003-2446-5577)
- X. Xu (ORCID: https://orcid.org/0000-0002-4302-1042)
- Yang Hu (ORCID: https://orcid.org/0000-0001-7826-045X)
- Jiayue Feng
- Xuena Yu (ORCID: https://orcid.org/0009-0002-1418-5678)
Institutions
- North West Agriculture and Forestry University (CN)
- Beijing Academy of Agricultural and Forestry Sciences (CN)
- Ministry of Agriculture (TN)
Publication Details
- Journal
- Journal of Integrative Agriculture
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1016/j.jia.2024.12.038
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00