Investigation Add Effect Hexane Extract of Juncus Rigidus Roots on Human Dermal Fibroblast Cell Line (DHSF)

Background: The study examined the effect of the hexane extract from Juncus rigidus roots on the human dermal fibroblast cell line (DHSF) with the aim of exploring and identifying safe therapeutic alternatives for normal cells. Materials and Methods: The active compounds (phenols and flavonoids) in the plant extract were quantified, yielding (15.87, 17.33) mg/g, respectively. The DPPH assay, using concentrations of (20, 30, 40) mg/ml, recorded inhibition rates of (21.3, 23.44, 26.24) %, respectively. The amount of reactive oxygen species (ROS) in the root extract was found to be very low, measuring 343 nml. The extract was tested on normal cells of the human DHSF line at five concentrations (25, 50, 100, 200,400) µg/ml and for three durations (24, 48, 72) hours. Results: The highest cytotoxicity rate was recorded at a concentration of 400 µg/ml, reaching 34.69% after 72 hours of exposure to the extract, with significant differences observed between the higher concentrations at a probability error rate of P ≤ 0.05. In contrast, lower concentrations showed less inhibition of the cells, with the lowest concentration of 25 µg/ml resulting in a minimal rate of 3.03%. No significant differences were observed between the lower concentrations at exposure times of 24 and 48 hours. Conclusion: The extract is relatively safe for normal DHSF cell lines even at the highest concentration used in the experiment, with the highest cell mortality rate being after three days of exposure.