Fluorescence-Based Simple and Practical Assay Method for DNA Damage Analysis in DNA-Encoded Library Synthesis
The dsDNA-selective fluorescent-dye-based DNA damage assay was developed for DNA-encoded library (DEL) synthesis. For the various DEL synthesis conditions, the assay was validated through cross-checking with high-performance liquid chromatography (HPLC) analysis, and the fact was confirmed that the usage of a specific ratio of organic solvent can critically induce DNA damage. Also, the applicability of the assay was confirmed through the screening of the DNA-damaging condition of the on-DNA amide coupling reaction and Pd-catalyzed on-DNA <i>N</i>-arylation reaction.
Authors
- Min Su Han (ORCID: https://orcid.org/0000-0001-9588-6980)
- Hohjai Lee (ORCID: https://orcid.org/0000-0002-6810-0206)
- Yeongcheol Ki (ORCID: https://orcid.org/0000-0002-4466-8470)
- Myo Win
- Seungyoon Kang (ORCID: https://orcid.org/0000-0001-6122-5041)
- Gyung A Kim
Institutions
- Gwangju Institute of Science and Technology (KR)
Publication Details
- Journal
- Bioconjugate Chemistry
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1021/acs.bioconjchem.4c00483
- Citations
- 1
- Primary Topic
- Chemical Synthesis and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- National Research Foundation of Korea