UV Spectrophotometric Studies of Ashwagandha, Chamomile and Fever Few Flowers Oil: Method Development and Validation

Using the UV-Vis spectrophotometry technique is a simple way to estimate or quantify any pharmacological compounds. Present research focused on development and validation of UV-visible method for feverfew (FFW), chamomile (CMM), and ashwagandha (AGA) flower oils. This method was also applied in conjunction with the UV spectrophotometric analysis of AGA, CMM, and FFW. Method validation parameters revealed that specificity, accuracy, linearity, precision and robustness met the acceptance criteria of ICH standards. It was concluded that developed. UV spectroscopic method proved to be sufficiently precise, robust, linear, and accurate for routine analysis of AGA, CMM, and FFW in polyherbal formulations.