Cardioprotective Activity of Nyctanthes arbor-tristis Linn Extract Loaded Nanoparticles

The study targets to develop and evaluate nanoparticles loaded with Nyctanthes arbor-tristis Linn stem extracts for its cardioprotective activity. The Nyctanthes-arbor-tristis Linn stem was extracted by continuous hot percolation method using the soxhlet apparatus and subjected to phytochemical evaluation. Nanoparticles was developed by precipitation technique with zinc nitrate and sodium hydroxide and characterized by laser light transmission test, scanning electron microscopy and dynamic light scattering. The safety profile was performed as per OECD 423 guidelines. The cardioprotective activity of novel nano-formulation has been investigated by in-vivo doxorubicin induced cardiotoxicity in rats. The percentage yield of Nyctanthes-arbor-tristis Linn stem extract was found to be 6.2% w/w and revealed the presence of various primary and secondary metabolites. The particle size of nanoparticles was in the range of 58.59 - 79.58nm with zeta potential value -37.38 and In-vitro drug release was 99.14%. Acute toxicity study didn’t show any toxicity and mortality. Hence 200mg/kg and 400mg/kg where selected for further studies. Nano formulation showed significant reduction in the cardiac biomarkers in the doxorubicin induced cardiotoxicity. ECG showed normal cardiac rhythm and histopathological study shown a normal architecture of the cardiac tissue in nanoparticle loaded Nyctanthes arbor tristis Linn extract treated animals. Based on the results obtained, the nanoparticle loaded Nyctanthes arbor tristis Linn stem extract found to have significant cardioprotective activity.