Effects of Nanopolystyrene on the Chlorophyll pigment content of Chlorella vulgaris

This study used a long-term toxicity test to investigate how the microalga Chlorella vulgaris responded to nanopolysteren exposure under different light spectrums. Our experiment involved subjecting algae to polystyrene doses of 0, 0.5, 5, and 10 mg/L. We design an incubator consisting of three sections, each offering a distinct red, blue, and white light spectrum, for the purpose of cultivating algal colonies. Chlorophyll-a and b were estimated at consistent time intervals (1, 4, and 10 days) during the 29-day incubation period, using three replicate samples for each treatment. Experimental results have demonstrated a reduction in both algal cell viability and chlorophyll concentration when exposed to (5 and 10 mg/l) of nanopolysterene. The findings of our study indicate that the growth and chlorophyll content of C. vulgaris are significantly affected by the red-light spectrum and with the increase of nanopolyesterne concentrations.