DEVELOPMENT OF AN INJECTABLE CHITOSAN-BASED BIOINK FOR ENHANCED BONE FRACTURE HEALING VIA MSC-DERIVED OSTEOGENESIS

Worldwide, bone fractures are a major public health concern and, there is an urgent requirement to create new treatments to accelerate the rate of the bone healing process. This research mainly relies on creating a hydrogel that satisfies the bone marrow mesenchymal stem cells (BM-MSCs) and makes it a suitable medium to differentiate into osteogenic cells. We combine the BM-MSCs with a chitosan polymer-based hydrogel, infused with PVA to enhance bone healing, this unique mixture forms " Bioink ". We induced a closed fracture model in the rat's right humerus, followed by injection of a single dosage of bioink. On day 14 with the weekly x-ray examination, we noticed complete bone closure although it was expected that for instance, adolescent rats, specifically those aged 6 weeks, are capable of forming bone to bridge the fracture gap within four weeks, so we terminated the experiment on day 14 as bioink, as observed, had reduced the healing time by half, which is a promising result. In conclusion, the current study's findings as a pilot test point out the possibility of this Bioink being involved in the treatment and acceleration of bone fractures.