Evaluation of Reference Gene Stability for Investigations of Intracellular Signalling in Human Cancer and Non-Malignant Mesenchymal Stromal Cells
We analysed and selected stable reference genes for RT-qPCR analysis in the wide panel of cancer cell lines and MSCs. The study provides a reliable tool for future research concerning the expression of genes involved in various intracellular signalling pathways and emphasises the need for careful selection of suitable references before analysing target gene expression.
Authors
- Vera Kosheverova (ORCID: https://orcid.org/0000-0002-9221-9362)
- М. В. Харченко (ORCID: https://orcid.org/0000-0001-9588-5072)
- Alexander Schwarz (ORCID: https://orcid.org/0000-0003-2707-1397)
- Rimma Kamentseva (ORCID: https://orcid.org/0000-0001-6923-1577)
- Е. С. Корнилова (ORCID: https://orcid.org/0000-0002-2951-6292)
Institutions
- Institute of Evolutionary Physiology and Biochemistry (RU)
- St Petersburg University (RU)
- Institute of Cytology (RU)
Publication Details
- Journal
- Frontiers in Bioscience-Scholar
- Published
- 2024-12-25
- DOI
- https://doi.org/10.31083/j.fbs1604026
- Citations
- 2
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Russian Science Foundation