<i>Grammatophyllum stapeliiflorum</i> Extract as a Suppressor of AGS Gastric Cancer Cell Growth

This study examined the anti-proliferative and anti-migratory effects of a Grammatophyllum stapeliiflorum extract (GSE) on AGS gastric cancer cells, with a focus on its potential mechanisms against tumor growth and metastasis. Gastric cancer is a highly aggressive malignancy associated with a poor prognosis, necessitating novel therapeutic strategies to improve the outcomes. In this research, AGS cells were treated with various GSE concentrations, and the cell viability was evaluated using a WST-1 assay at multiple time points. The results revealed the dose-dependent inhibition of cell proliferation, with higher GSE concentrations leading to significantly reduced viability. A wound healing assay was performed to explore the effects of GSE, showing that it effectively inhibited AGS cell migration, indicating anti-metastatic properties. In addition, real-time PCR analysis identified the downregulation of several key oncogenes, including KRAS, BCL-2, JAK2, and C-Myc, in GSE-treated cells compared to the untreated controls. These oncogenes play crucial roles in tumor growth, cell survival, and metastasis. These findings suggest that GSE has anti-cancer effects by suppressing cell proliferation and migration in gastric cancer cells. These results highlight the potential of GSE as a promising natural therapeutic agent, warranting further investigation to elucidate its mechanisms and therapeutic applicability in vivo.