Evaluation of 2-formylphenylboronic and 3-chlorophenylboronic acid derivatives for in vitro cytotoxicity and cell migration

Wound treatment and skin regeneration is a complex process, and non-healing wounds pose a major socioeconomic burden in terms of health. Effective and alternative approaches are needed for successful wound management. Although boranic acid derivatives have been reported to have positive and strong effects on wound healing, phenyl substituted boranic acid derivatives can be used as more regenerative and effective compounds in healing. In this study, the in vitro cytotoxic effects of 2-formyl phenyl boronic acid and 3-chloro phenyl boronic acid on L929 fibroblast cell lines were investigated by WST-8 analysis and their wound healing effects were investigated by cell migration test. Our data reveal concentration-dependent effects of both boranic acid derivatives. For 2-formyl phenyl boronic acid, dosage applications between 3.90-31.25 µg/ml showed a viability of 84 % and above, and at higher concentrations the viability was found to be 5-10 %. For 3-chloro phenyl boronic acid, a viability rate of 64-109 % is observed as a result of dosage applications between 3.90-250 µg/ml, while the percentage of viability decreases to 17 % at a concentration of 500 µg/ml. Cell migration test data show that the effects of phenyl boronic acid (PBA) derivatives in terms of cell migration increase as time increases, and the effect of 2-formyl phenyl boronic acid at the 24th hour is quite effective in terms of cell migration. Since the wound healing effect of PBA derivatives is concentration dependent, it should be taken into consideration that the use of high concentrations may be toxic.