Dynamic Metabolomic Changes in the Phenolic Compound Profile and Antioxidant Activity in Developmental Sorghum Grains
Phenolic compounds (PC) were analyzed by UHPLC-ESI-QTOF-MS<sup>E</sup> in two sorghum genotypes, harvested in two growing seasons (GS) at five distinct days after flowering (DAF) to evaluate how genotype/GS influences the PC synthesis and antioxidant capacity during grain growth. Total phenolic contents were strongly correlated with antioxidant capacity (<i>r</i> > 0.9, <i>p</i> < 0.05). Globally, 97 PC were annotated, including 20 PC found irrespective of the grain developmental stage and genotype/GS. The phenolic profile clearly differs between stages: phenolic acids were the most abundant class in early stages (50%), and flavonoid accumulation becomes predominant in late ones (3/5 of total ion abundance). Dimeric and trimeric tannins were identified even in 10DAF grains. Chemometry revealed great PC variability between genotypes (27%) and important biomarkers of GS differentiation (e.g., ferulic acid). This work can input open databases of PC and paves the way to understand biosynthetic pathways of PC in sorghum and future sorghum selection.
Authors
- Carolina Thomaz dos Santos D’Almeida (ORCID: https://orcid.org/0000-0003-4768-9071)
- Hamza Mameri (ORCID: https://orcid.org/0000-0001-7762-9542)
- Mariana Simões Larraz Ferreira (ORCID: https://orcid.org/0000-0002-6043-0921)
- Nancy Terrier (ORCID: https://orcid.org/0000-0002-0959-4701)
- Marie‐Hélène Morel
Institutions
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
- Université de Montpellier (FR)
- Ingénierie des Agropolymères et Technologies Emergentes (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- IONICS Mass Spectrometry (Canada) (CA)
- Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales (FR)
- Institut Agro Montpelier (FR)
- Impact (CA)
- Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (FR)
- Universidade Federal do Estado do Rio de Janeiro (BR)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2024-12-30
- DOI
- https://doi.org/10.1021/acs.jafc.4c08975
- Citations
- 4
- Primary Topic
- GABA and Rice Research
- Type
- article
- Field-Weighted Citation Impact
- 2.40
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
- Universidade Federal do Estado do Rio de Janeiro