CRISPR-associated Plasmonic Colorimeter Method (Ca-PCM): A real-time RGB detection system for gold nanoparticles-based nucleic acid biosensors
We have shown that the combination of RGB analysis and continuous temporal measurements is a novel and promising method to characterise the behaviour of gold nanoparticle-based biosensors and to achieve dose-dependent target detection. In addition, the simplicity and cost-effectiveness of this new approach expand the possibilities of other plasmonic-based biosensors and their applicability in low-resources clinical environments.
Authors
- Rodolfo Miranda (ORCID: https://orcid.org/0000-0002-1064-6724)
- Isidoro Martínez (ORCID: https://orcid.org/0000-0002-5821-4840)
- Carmen Escalona‐Noguero (ORCID: https://orcid.org/0000-0003-4365-0911)
- Begoña Sot (ORCID: https://orcid.org/0000-0001-8763-0651)
- Milagros Castellanos (ORCID: https://orcid.org/0000-0003-2526-5323)
- Hernán Alarcón-Iniesta
- Daniel Granados (ORCID: https://orcid.org/0000-0001-7708-9080)
- Demián Pardo (ORCID: https://orcid.org/0000-0002-0251-0704)
- Rafael Cantón (ORCID: https://orcid.org/0000-0003-1675-3173)
- Ciro Rodríguez Díaz (ORCID: https://orcid.org/0000-0002-8271-2495)
- J. Julio Camarero (ORCID: https://orcid.org/0000-0003-2436-2922)
- Ramón Bernardo Gavito (ORCID: https://orcid.org/0000-0001-6881-2599)
- Andrés Valera (ORCID: https://orcid.org/0000-0002-2479-7208)
- María López‐Valls (ORCID: https://orcid.org/0000-0001-9455-508X)
- Juan Carlos Galán (ORCID: https://orcid.org/0000-0002-8447-0960)
- G. Arana (ORCID: https://orcid.org/0000-0001-9489-6386)
- Javier De Vicente
- W. S. Smith
- Héctor Guerrero
- Sergio de Las Heras
- Sara Cobelo
Institutions
- Madrid Institute for Advanced Studies (ES)
- Instituto de Salud Carlos III (ES)
- Instituto Ramón y Cajal de Investigación Sanitaria (ES)
- Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (ES)
- Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (ES)
- Universidad Autónoma de Madrid (ES)
- Universidad Politécnica de Madrid (ES)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2024-12-31
- DOI
- https://doi.org/10.1016/j.aca.2024.343601
- Citations
- 5
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- Comunidad de Madrid
- Ministerio de Ciencia, Innovación y Universidades
- Ministerio de Economía y Competitividad