In vitro Antiproliferative Activity of Lissotriton schmidtleri (Raxworthy, 1988) Skin Secretion on Human Breast Cancer (MCF7) Cell Line

: In this study, the in vitro antiproliferative activity of Lissotriton schmidtleri skin secretion on estrogen-sensitive human breast cancer (MCF7) cells was reported for the first time. The effects of L. schmidtleri skin secretion at concentrations of 0.5, 5, and 50 μg/mL on the MCF7 cell line were evaluated using the MTT assay after 48 hours of incubation. According to the MTT assay results, L. schmidtleri skin secretion inhibited MCF7 cell viability by approximately 64% at a concentration of 50 μg/mL, with an IC50 value calculated as 20.81 ± 0.87 μg/mL. Based on these findings, it is suggested that L. schmidtleri skin secretion may serve as a potential anticancer agent against breast cancer.