How do manipulation checks interfere with the inference of causal relationships?

This study investigates the performance of mediation analyses, including manipulation check variables, in experimental studies where manipulated psychological attributes are independent variables. We simulated the level of manipulation intensities and measurement errors of the manipulation check variable to test the validity of the analytic practice. Our results showed that when manipulation is successful and measurement error is low, mediation analyses with the manipulation check variable revealed an unstable path coefficient and standard error. Moreover, many of the detected indirect effects were inconsistent mediation situations. However, when individual differences in psychological attributes remained within the condition (low manipulation intensity) and the manipulation check variable contained low measurement error, the indirect effect indicated the validity of the manipulation. We discuss the implications of our findings for the use of manipulation checks in experimental research.