Amphiphilic dynamic covalent polymer vectors of siRNA
Dynamic covalent polymers (DCPs) recently emerged as smart siRNA delivery vectors, which dynamically self-assemble through siRNA templating and depolymerize in a controlled manner. Herein, we report the dynamic combinatorial screening of cationic and amphiphilic peptide-based monomers. We provide experimental evidence, by mass spectrometry analyses, of the siRNA-templated formation of DCPs, and show that amphiphilic DCPs display superior activity in terms of siRNA complexation and delivery in cells. Thus, the work describes a new type of siRNA vector based on dynamic covalent lipopolyplexes, which feature improved activity as well as better nano-structuration compared to previous generations of DCPs.
Authors
- Jeanne Leblond Chain (ORCID: https://orcid.org/0000-0001-8390-4811)
- Sachin Dattram Pawar (ORCID: https://orcid.org/0000-0002-9085-029X)
- Emmanuelle Sachon (ORCID: https://orcid.org/0000-0002-2271-8924)
- Nadir Bettache (ORCID: https://orcid.org/0000-0001-5426-7024)
- Yannick Bessin
- Laure Lichon
- Sébastien Ulrich (ORCID: https://orcid.org/0000-0002-6080-3345)
- José García Coll (ORCID: https://orcid.org/0000-0002-6012-7287)
- Pauline Trousselier
Institutions
- École Nationale Supérieure de Chimie de Montpellier (FR)
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Inserm (FR)
- Université de Montpellier (FR)
- Université Paris Sciences et Lettres (FR)
- École Normale Supérieure - PSL (FR)
- Sorbonne Université (FR)
- Institut des Biomolécules Max Mousseron (FR)
- Laboratoire des Biomolécules (FR)
- Acides nucléiques: Régulations naturelles et artificielles (FR)
Publication Details
- Journal
- Chemical Science
- Published
- 2024-12-30
- DOI
- https://doi.org/10.1039/d4sc07668k
- Citations
- 7
- Primary Topic
- RNA Interference and Gene Delivery
- Type
- article
- Field-Weighted Citation Impact
- 1.64
Funders
- Agence Nationale de la Recherche
- Fondation ARC pour la Recherche sur le Cancer
- Université de Montpellier
- Sorbonne Université