Evaluating the sensitivity of a chronic plant bioassay relative to an independently derived predicted no-effect thresholds to support risk assessment of very hydrophobic organic chemicals

Environmental risk assessments of very hydrophobic organic compounds (VHOCs) in soils are often difficult because multiple processes (e.g., sorption, volatilization, biodegradation) can complicate the interpretation of results. A standardized soil dosing and aging procedure is presented for assessing bioavailability of VHOCs in a synthetic soil, which was used to evaluate the phytotoxicity of VHOCs. The soil preparation protocol resulted in relatively stable freely dissolved concentrations of test substance compared to bulk soil concentrations with some losses likely due to volatility and biodegradation. This dosing method was used in a chronic terrestrial plant toxicity bioassay to evaluate the potential toxicity of VHOCs on complex reproductive endpoints like inflorescence and seed bud formation. Testing included representative hydrocarbons and three very hydrophobic lubricant substances (logKow > 10). The toxicity data were used to evaluate existing predicted no-effect concentrations (PNECs) that had originally been derived with the target lipid model, which did not have these higher order chronic plant endpoints. The initial exposure concentrations were set at the PNECs to provide an independent validation of the PNEC. This evaluation was performed to expand the domain of applicability of the PNEC to VHOCs and for the chronic terrestrial plant endpoints. No effects were observed on plant biomass or inflorescence production at these low exposure concentrations, demonstrating that the established PNEC is protective of long-term plant health. The results of the present study confirm that the new dosing method is fit for purpose, and that the existing PNEC framework can be extended to chronic plant endpoints for VHOCs.