Identification of blaOXA−51−23−58, blaVIM, blaNDM, and blaIMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients

In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla<sub>OXA-51</sub>, bla<sub>OXA-23</sub>, bla<sub>OXA-58</sub>, bla<sub>NDM</sub>, bla<sub>IMP</sub>, and bla<sub>VIM</sub> genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla<sub>OXA-23</sub> gene, 23 (92.0%) contained the bla<sub>OXA-51</sub> gene, and 1 (4.0%) isolate carried the bla<sub>NDM</sub> gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla<sub>OXA-51</sub> and bla<sub>OXA-23</sub> genes (oxacillinase genes) were dominant genes, but bla<sub>IMP</sub> and bla<sub>OXA-58</sub> were not local carbapenem resistant genes in Isfahan.

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Journal
BMC Research Notes
Published
2024-12-27
DOI
https://doi.org/10.1186/s13104-024-07047-5
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5
Primary Topic
Antibiotic Resistance in Bacteria
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article
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0.85
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article

Identification of blaOXA−51−23−58, blaVIM, blaNDM, and blaIMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients

Yasaman Ahmadi, Dariush Shokri, Seyed Mahdi Ghasemi, Kosar Salmani Nasrabadi
5 citations
BMC Research Notes
Antibiotic Resistance in Bacteria
0.85
article

Identification of blaOXA−51−23−58, blaVIM, blaNDM, and blaIMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients

Yasaman Ahmadi, Dariush Shokri, Seyed Mahdi Ghasemi, Kosar Salmani Nasrabadi
article en
5 citations

Abstract

In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla<sub>OXA-51</sub>, bla<sub>OXA-23</sub>, bla<sub>OXA-58</sub>, bla<sub>NDM</sub>, bla<sub>IMP</sub>, and bla<sub>VIM</sub> genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla<sub>OXA-23</sub> gene, 23 (92.0%) contained the bla<sub>OXA-51</sub> gene, and 1 (4.0%) isolate carried the bla<sub>NDM</sub> gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla<sub>OXA-51</sub> and bla<sub>OXA-23</sub> genes (oxacillinase genes) were dominant genes, but bla<sub>IMP</sub> and bla<sub>OXA-58</sub> were not local carbapenem resistant genes in Isfahan.

BMC Research NotesVol. 17(1)
Islamic Azad University, Tehran (IR), Isfahan University of Medical Sciences (IR), Isfahan University of Technology (IR)
Openalex Percentile: Top 29%
Antibiotic Resistance in Bacteria
0.85
DOIOpen Access PDFOpenAlex
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Identification of blaOXA−51−23−58, blaVIM, blaNDM, and blaIMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients — Yasaman Ahmadi, Dariush Shokri, et al. · BMC Research Notes (2024) | TGRS Research Map | TGRS