Renal Function Indices of Ulcerated Rats Pre-Treated with Binary Combinations of Persea americana Seed and Bryophyllum pinnatum Leaf Ethyl Acetate Fraction

The use of certain medications and herbal remedies poses a significant risk to renal function. The study aimed to investigate the effect of binary combinations of Persea americana seed (PAEF) and Bryophyllum pinnatum leaf (BPEF) ethyl acetate fractions on renal function indices of ulcerated rats. Wistar rats were divided into ten groups of five rats each. The groups were designated: normal control (NC) (Group 1), ulcer control (UC) (Group 2), Omeprazole (OMEP) (Group 3), PAEF (Group 4), BPEF (Group 5), PAEF + BPEF (1:1) (Group 6), PAEF + BPEF (1:2) (Group 7), PAEF + BPEF (1:3) (Group 8), PAEF + BPEF (2:1) (Group 9), and PAEF + BPEF (3:1) (Group 10). Groups 4-10 were pre-treated with 400 mgkg-1 body weight/day of ethyl acetate fractions of the plants, and their binary combinations orally for 21 days. On the 22nd day, after 12 h fasting, gastric ulcer was induced in the rats in groups 2-10 by a single oral dose of indomethacin (30 mg/kg body weight). Results showed that the administration of PAEF + BPEF (1:2), PAEF + BPEF (1:3), PAEF + BPEF (2:1), PAEF + BPEF (3:1), and omeprazole caused significant (p<0.05) elevation of serum urea when compared to NC and UC. The binary combinations PAEF + BPEF (1:1), PAEF + BPEF (1:3), PAEF + BPEF (2:1), PAEF + BPEF (3:1) caused normalization of serum calcium, magnesium, and zinc concentrations comparable to normal control. Therefore, the binary combinations of PAEF and BPEF may distort kidney function and electrolyte balance.