Evaluation of 8-Oxoguanine DNA Glycosylase-1(OGG1) Serum Levels in Patients with Type2 Diabetes Mellitus

: Chronic hyperglycemia in type 2 diabetes mellitus leads to elevated oxidative stress. As a consequence, the accumulation of reactive oxygen species (ROS)may cause additional damage to various biological macromolecules, including DNA. The current study aims to evaluate the DNA damage in type2 diabetic patients from Wasit Province by estimating the levels of 8-OHdG using ELISA. All samples were collected from the local community of Wasit province, Iraq. Forty-five type 2 diabetes mellitus patients (22 males and 23 females) and 35 healthy controls (17 males and 18 females) were genotyped for 8-oxoguanine DNA glycosylase-1(OGG1). Determination of 8-oxoguanine DNA glycosylase-1(OGG1) in sera of patients with T2DM and controls was done by using an Enzyme-linked immunosorbent assay ELISA.The results reveal highly significant differences, the OGG1 levels in the diabetic patients were higher than that of controls (646.96±2.14), controls (326.01±16.66), P= 0.0001. The OGG1 levels were elevated significantly among males and female’s patients withT2DM compared to the control group, 627.02±66.31 versus 679.68±63.27), P= 0.01 (381.85±29.33 versus 355.44±31.45), P = 0.0006, respectively. No significant differences were observed when comparing OGG1 levels between male and female patients with type2 diabetes, as well as in the control group: 627.03±66.31 versus 679.68±63.27, P=0.5974;381.85±29.36 vs.355.44±31.45, P=0.5478 in patients and controls respectively.679.68267±63.27413 versus627.02830±66.29612 with a non-significant difference P=0.5974. In conclusion levels of serum OGG1 are associated with diabetes mellitus