A Successful Approach to Extracted Human mtDNA and Amplify some Genes from FFPE Samples

Tissues that have been paraffin-embedded and fixed in formalin (FFPE) are a significant diagnostic resource for molecular and pathophysiological methods of cancer and numerous other illnesses. The most difficult obstacle is obtaining high-quality of DNA from FFPE tissues, especially mitochondrial DNA (mtDNA), as formalin fixation significantly compromises the integrity of DNA. To extract mtDNA, the Geneaid gSYNCTM DNA kit (cat#GS100) was used with fifty breast cancer tissues (block). To check the mtDNA quality, specific primers for the following genes, ATP6 gene (8527-9024) and D-Loop region (256-16277), were used to run a PCR reaction. Our data showed that the mtDNA was successfully extracted with high quantity and quality. The two genes were amplified via PCR with very high rate (97% average). In conclusion, the method described here has many possibilities for applications in various molecular research projects involving FFPET substances. It can be used to obtain a high quantity and quality of mtDNA from FFPE samples for different molecular diagnostic and research methods such as PCR, qPCR, Sanger sequencing, and whole genome sequencing.