Molecular Detection of Genes Related to Antimicrobial Resistance harbored on chromosome by PCR assay in P. aeruginosa

Forty-two typical P. aeruginosa strains were chosen, and Eight antibiotic-resistance genes linked to a spontaneous outbreak of the bacteria were analyzed (the same strains that were tested for the antimicrobial susceptibility). The findings revealed that 15 of the examined bacteria had the -lactamase gene on chromosomes with amplicons that were 264 bp in size. 21 tested strains harbored fusA1 & fusA2 gene on chromosome while 12 strains harbored orfN gene on chromosome . ropN gene found on chromosome in 10 strains . 16 strains harbored ampC gene on chromosome, 8 tested strains harbored fusA1 & fusA2 gene on chromosome, rpsJ gene found on chromosome in 2 strains. ptsP gene found on chromosome in 13 strains.