Generation of a PDK-1 knockout human embryonic stem cell line by CRISPR/(WAe009-A-2K) Cas9 editing
Pyruvate Dehydrogenase Kinase1 (PDK1) belongs to the family of kinases, regulates diverse metabolic processes. PDK1 is a susceptibility locus for heart failure via thinning of ventricle walls, and enlarged atria and ventricles. We successfully developed a PDK1 knockout (PDK1<sup>-</sup>/<sup>-</sup>) human embryonic stem cell (hESC) line using an episomal vector-based CRISPR/Cas9 system explore the role of PDK in human heart development. This PDK1-KO hESC line-maintained stem cell-like morphology, pluripotency, and normal karyotype and can differentiate into all three germ layers in vivo. This cell line will be a valuable tool for future research on the role of PDK1 in heart development.
Authors
- Hongfeng Jiang (ORCID: https://orcid.org/0000-0002-9261-0433)
- Muhammad Khawar Abbas (ORCID: https://orcid.org/0000-0001-7804-0748)
- Amina Saleem (ORCID: https://orcid.org/0000-0002-3064-4750)
- Jiaqi Fan (ORCID: https://orcid.org/0000-0003-3317-531X)
- Mingyu Wei
- Siyao Zhang (ORCID: https://orcid.org/0009-0006-0027-8776)
- Yang Xian (ORCID: https://orcid.org/0009-0004-1565-7205)
Institutions
- Capital Medical University (CN)
- Peking University (CN)
- Beijing Anzhen Hospital (CN)
- Peking University Third Hospital (CN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2024-12-28
- DOI
- https://doi.org/10.1016/j.scr.2024.103642
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- National Natural Science Foundation of China