HEPATOPROTECTIVE ACTIVITY OF MAGNESIUM BIS-ACETAMINOETHANESULFONATE IN KETATOCONAZOLE DRUG-INDUCED LIVER INJURY (EXPERIMENTAL STUDY)

The hepatoprotective activity of magnesium bis-acetaminoethanesulfonate with the laboratory code LBK-527 was studied in ketoconazole-induced liver injury. Drug-induced liver injury was modeled by intragastric administration of ketoconazole at a dose of 100 mg for 3 days. LBK-527 and the comparison drug heptral were administered to animals at a dose of 100 mg / kg / day and 60 mg / kg / day intraperitoneally 1 hour before the introduction of hepatotoxin for 14 days. Biochemical blood parameters characterizing the functional activity of the liver were studied, its histological picture of the liver was assessed, and macro- and micromorphometry of the organ was performed on the 7th and 14th days. The substance LBK-527 prevents the cytotoxic effect of ketoconazole on the functional activity of the liver. There is a decrease in ketoconazole-induced cytolysis, normalization of the level of total bilirubin in the blood plasma of animals. The appearance of the liver, its relative and absolute weight in animals with drug-induced hepatitis treated with LKB-527 were comparable with intact rats. On histological preparations stained with hematoxylin and eosin, a decrease in the area of necrosis of the liver parenchyma was observed, the beam structure of the tissue was preserved. The area of the cytoplasm, nucleus and nuclear-cytoplasmic ratio approached the values of the intact group. The appearance of multinucleated hepatocytes indicated the activation of the synthetic activity of the liver and the launch of regeneration mechanisms. Thus, it can be concluded that the substance under study has a hepatoprotective effect.