Complete Genome of Multidrug-Resistant <i>Enterococcus faecalis</i> JF4A2234 Belonging to Sequence Type 620

Enterococci are Gram-positive, facultative anaerobic bacteria that inhabit the gastrointestinal tract of various organisms, including humans [1].To date, more than 50 species are known with Enterococcus faecalis and Enterococcus faecium accounting for more than 80% of clinical isolates and being most common in the intestines of humans and animals [2,3].Differences in antibiotic resistance among enterococci from various farm animals are known to be one of the important factors in determining the level of resistance based on the use of antibacterial agents [4].E. faecalis strain JF4A2234 was isolated from a fecal swab sample from a 15-week-old pig in South Korea.The rectal swab collected from the pig was suspended in brain heart infusion broth (BD Bioscience, USA).The inoculum was streaked on enterococcosel agar (BD Bioscience, USA) and incubated at 37 for 48 h.Suspected enterococci colonies, which were translucent with brownish-black to black zones, were cultured on blood agar plates (Synergy Innovation, South Korea).Subsequently.E. faecalis was identified by PCR and sequencing analysis using the housekeeping gene gdh (5'-GGCGCACTAAAAGATATGGT-3' and 5'-CCAAGATTGGGCAACTTCGTCCCA-3'), and the nucleotide sequences of the 16S rRNA gene were determined from a species-specific PCR-positive colony.The strain E. faecalis JF4A2234 exhibited 100% similarity to E. faecalis strain ATCC 19433 (GenBank accession No. ASDA01000001.1)when compared using the 16S rRNA sequence in EzBioCloud database [5].The multilocus sequence type (ST) of strain E. faecalis JF4A2234 was identified as ST620 using the E. faecalis scheme in PubMLST (https://pubmlst.org/organisms/enterococcus-faecalis).Disk diffusion and broth microdilution methods were employed for an antibiotic susceptibility test following the Clinical and Laboratory Standards Institute guidelines.Strain JF4A2234 was resistant to various antibiotics, such as fluoroquinolone (ciprofloxacin), aminoglycoside (gentamicin, streptomycin), tetracycline Enterococcus faecalis strain JF4A2234 was isolated from the feces of a pig in the Republic of Korea.The complete genome of E. faecalis JF4A2234 was obtained through a hybrid assembly of long-and short-read sequences.It consisted of one chromosome (2,796,215 bp with 37.7% GC content) and one circular plasmid (92,100 bp with 34.6% GC content).The annotation process identified 2,803 protein-coding sequences, 12 rRNA genes, and 62 tRNA genes.The genome harbored multiple antibiotic resistance genes, such as aac(6")-le-aph(2")-Ia, aad(6), ant(6)-Ia, ant(9)-Ia, aph(3')-IIIa, tetM, tetL, ermB, dfrG, cat, sat-4, IsaE, and lnuB, which confer resistance to aminoglycoside, tetracycline, chloramphenicol, trimethoprim, erythromycin, lincosamide, and streptothricin.