The effect of physical and chemical mutagenesis in lentil (Lens culinaris Medik.)

This comprehensive study investigates the impact of physical and chemical mutagenesis on lentil (Lens culinaris Medik.) to enhance genetic variability and identify mutants with improved agronomic traits. Gamma radiation and ethyl methanesulfonate (EMS) were used as mutagens. The experimental design, treatment doses, and observed morphological, physiological, and biochemical responses of lentil genotypes are presented. The results demonstrate significant variability in traits such as plant height, seed yield, chlorophyll content, biological yield, harvest index, and 1000-seed weight, with notable differences between control and treated groups. The findings highlight the potential of mutagenesis for crop improvement and genetic diversity enhancement in lentil breeding programs. Statistical analysis revealed significant correlations among key traits, and path coefficient analysis was used to identify traits with the most direct impact on seed yield.