Qualitative and Quantitative Analysis of Synthetic Drug Content Of Sodium Diclofenac In Rheumatic Herbal Medicine From Wonosobo City

The use of traditional medicine by a relatively high population, provides an opportunity for manufacturers to undertake counterfeiting of conventional medicine. The purpose of this research is to identify the presence of the Medicinal Chemical Substance (BKO) sodium diclofenac in rheumatic herbs in the city of Wonosobo so that it can be a reference related to the safety of the traditional medicine consumed. The samples used were five brands, AR, AT, WM, AN, S, which were obtained from a herbs store in the Wonosobo area. Identification of BKO using thin-layer chromatography (KLT) and UV-Vis spectroscopic photometry at wavelengths of 282 nm. Qualitative analysis using silica gel GF254 still phase, and toluene mixture motion phase: ethyl acetate: glacial acetic acid (60:40:1). Detection was done with 254 nm and 366 nm UV rays. The results showed a positive AT sample containing diclofenac sodium with a value of Rf=0.75 equal to the standard Rf sodium diclophenac. The default curves for rate determination using spectroscopic photometry are made at 5, 10, 15, 20 and 25 ppm. The sodium content of diclofenac in the AT replication 1 sample was 2.23%, the replication 2 sample contained 1.56%, and the replica 3 AT sample 1.41%.