An Optimized Method for Single Cell Cloning of Human CAR-T Cells based on FBS-coated Plates
This new approach is efficient, economical, and scalable for isolating specific homogenous T cells and promoting their clonal proliferation and expansion. Furthermore, this method improves T cell adherence, proliferation, and functional effectiveness, offering a potential foundation for advancing CAR-T cell therapies aimed at solid tumors. Future research should concentrate on optimizing culture conditions and testing this method in preclinical animal models to ensure its clinical applicability and efficacy.
Authors
- Mahdie Jafari
- Masoud Moghaddam Pour (ORCID: https://orcid.org/0000-0002-5307-7226)
- Zahra Sharifzadeh (ORCID: https://orcid.org/0000-0002-4419-0315)
- Mohammad Ali Shokrgozar (ORCID: https://orcid.org/0000-0001-5123-4155)
- Shahriyar Abdoli (ORCID: https://orcid.org/0000-0002-4052-332X)
Institutions
- Pasteur Institute of Iran (IR)
- Razi Vaccine and Serum Research Institute (IR)
- Golestan University (IR)
- Iran Banking Institute (IR)
- Golestan University of Medical Sciences (IR)
Publication Details
- Journal
- Advanced Pharmaceutical Bulletin
- Published
- 2024-12-26
- DOI
- https://doi.org/10.34172/apb.43798
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Iran National Science Foundation
- Pasteur Institute of Iran