Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma
Primary central nervous system diffused large B-cell lymphoma (PCNS-DLBCL) is a rare type of non-Hodgkin lymphoma restricted to the central nervous system (CNS). To explore its specific pathogenesis and therapeutic targets, we performed multi-omics sequencing on tumor samples from patients diagnosed with PCNS-DLBCL, secondary CNS-DLBCL or extracranial (ec) DLBCL.By single-cell RNA sequencing, highly proliferated and dark zone (DZ)-related B cell subclusters, MKI67_B1, PTTG1_B2 and BTG1_B3, were predominant significantly in PCNS-DLBCL. Compared to SCNS-DLBCL and ecDLBCL, an immune-suppressive tumor microenvironment was observed in PCNS-DLBCL by analysis of immune-stimulating/inhibitory ligand‒receptor (L-R) pairs. By performing whole-exome sequencing in 93 patients, mutations enriched in BCR-NFkB and TLR pathways and the cooperation of these two pathways were found to be predominant in PCNS-DLBCL comparing to nonGCB-ecDLBCL. In summary, our study provides comprehensive insights into the transcriptomic and genetic characteristics of PCNS-DLBCL in contrast to ecDLBCL and will help dissect the oncogenic mechanism of this disease.
Authors
- Jiajun Zheng (ORCID: https://orcid.org/0000-0001-5005-9682)
- Kunpeng Wu (ORCID: https://orcid.org/0000-0001-6408-8927)
- Zonghua Wang (ORCID: https://orcid.org/0000-0002-0869-9683)
- Kang H. Zheng (ORCID: https://orcid.org/0000-0001-8954-1337)
- Yuqi Zhu
- Hong Chen (ORCID: https://orcid.org/0000-0003-1904-0857)
- Jianping Song (ORCID: https://orcid.org/0000-0003-3411-1457)
- Fengping Zhu (ORCID: https://orcid.org/0000-0002-5976-8861)
- Wei‐Qun Ding (ORCID: https://orcid.org/0000-0001-7358-3700)
- Rui Feng (ORCID: https://orcid.org/0000-0003-3895-8989)
- Dongxiao Zhuang (ORCID: https://orcid.org/0000-0002-4964-6529)
- Bo Dai (ORCID: https://orcid.org/0000-0001-7861-1344)
- Yifan Yuan (ORCID: https://orcid.org/0000-0001-8645-3112)
- Yan Yuan (ORCID: https://orcid.org/0000-0002-3064-1955)
- Tianling Ding (ORCID: https://orcid.org/0000-0002-5727-4761)
- Haoshu Zhong
- Hong Ding (ORCID: https://orcid.org/0000-0002-9998-0904)
- Hongmei Wu (ORCID: https://orcid.org/0000-0002-1402-4359)
- Yuan Li (ORCID: https://orcid.org/0000-0002-5550-0307)
- Qian Wang (ORCID: https://orcid.org/0000-0002-2149-384X)
- Tong Chen (ORCID: https://orcid.org/0000-0001-7895-2077)
- Jingyuan Xiang (ORCID: https://orcid.org/0000-0002-1755-7978)
- Xiaodong Gu (ORCID: https://orcid.org/0009-0006-5088-1127)
- Ying Mao (ORCID: https://orcid.org/0000-0001-8055-115X)
- Liqian Xie
Institutions
- Fudan University (CN)
- Shanghai Stomatological Hospital (CN)
- Huashan Hospital (CN)
Publication Details
- Journal
- Neoplasia
- Published
- 2024-12-28
- DOI
- https://doi.org/10.1016/j.neo.2024.101119
- Citations
- 2
- Primary Topic
- CNS Lymphoma Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.74