The fermentation of <i>Cordyceps militaris</i> polysaccharide influenced gut bacterial LPS structure formation and changed its antigenicity

Gut bacterial lipopolysaccharide (LPS) could be released into the circulatory system via the gut-liver axis and cause inflammatory immune response, while Cordyceps militaris polysaccharide (CMP40) has been reported to be effective in alleviating this inflammatory response. In this study, the effects of CMP40 gut fermentation on internal LPS structure formation and the subsequent immune response were explored. Results showed that CMP40 could change antigenicity of LPS of Vibrio parahaemolyticus, Salmonella enterica, and enterotoxigenic Escherichia coli, indicated by a reduced level of NO, IL-1β, IL-6, and TNF-α. The LPS structure of these three strains were further elucidated. ESI/MS results revealed that CMP40 fermentation could alter the LPS structure by removing phosphate group from a single Kdo sugar or removing additional sided fatty acid chain. The gene expressions of enzymes that are responsible for group transfer further confirmed this structure modification process. This study focused on the regulation of polysaccharide on gut bacteria LPS and provided a new insight into health effect of CMP40.