Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants
COE-3 adsorption on both TMOS or UV-TMOS surface was irreversible. However, nonionic surfactant PS80 could partially remove pre-adsorbed COE-3 from these surfaces, forming a co-adsorption layer. Interestingly, while the hydrophobic TMOS surface prevented mAb adsorption when pre-treated with PS80, the amphiphilic UV-TMOS did not. Furthermore, when COE-3 and PS80 were injected as a mixture, PS80 formed a preventative layer on both surfaces against COE-3 adsorption. These results highlight the significance of surface hydrophobicity in controlling mAb adsorption in the presence of nonionic surfactants.
Authors
- Zeyuan Zhuang
- Kangcheng Shen (ORCID: https://orcid.org/0000-0003-0817-9140)
- Mingrui Liao (ORCID: https://orcid.org/0000-0002-9481-4026)
- Stephen C. L. Hall (ORCID: https://orcid.org/0000-0003-0753-5123)
- Jian R. Lu (ORCID: https://orcid.org/0000-0001-5648-3564)
- Cavan Kalonia (ORCID: https://orcid.org/0000-0002-4028-0065)
- Xuzhi Hu (ORCID: https://orcid.org/0000-0002-9246-7040)
- Kai Tao (ORCID: https://orcid.org/0000-0003-3899-5181)
- Haoran Wu (ORCID: https://orcid.org/0000-0001-5822-8698)
- Zongyi Li (ORCID: https://orcid.org/0000-0002-5989-9132)
- Renzhi Li (ORCID: https://orcid.org/0000-0002-3674-7777)
Institutions
- Rutherford Appleton Laboratory (GB)
- University of Manchester (GB)
- Lanzhou Institute of Chemical Physics (CN)
- AstraZeneca (United States) (US)
- Zhejiang University (CN)
Publication Details
- Journal
- Journal of Colloid and Interface Science
- Published
- 2024-12-28
- DOI
- https://doi.org/10.1016/j.jcis.2024.12.214
- Citations
- 1
- Primary Topic
- Protein purification and stability
- Type
- article
- Field-Weighted Citation Impact
- 0.23