Applications of CRISPR/Cas9 systems for gene editing in Bacillus spp.

CRISPR/Cas (Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) is an immune system in prokaryotic organisms that plays a role in defending against foreign genetic elements, such as plasmid invasion and bacteriophage attacks.The key event occurred in 2013 when this technology was identified as capable of editing human genes.When the CRISPR/Cas system was discovered to have the ability to edit human genes in 2013, an increasing number of studies have worked to improve the system with the goal of applying gene editing to a variety of subjects, including microorganisms, plants, animals, and humans.Since then, this system has continuously been optimised, offering many advantages such as ease of design and use, along with superior accuracy compared to previous gene editing methods.Recently, gene editing in Bacillus species using this system has attracted a lot of attention because it offers a wide range of applications in agriculture, industry, and medicine.This article presents information on the CRISPR/Cas9 system and its application in the gene editing of Bacillus species.This information helps scientists easily access the CRISPR/Cas9 geneediting system, facilitating its application in their research more effectively and conveniently.