METHOD DEVELOPMENT AND VALIDATION OF RILPIVIRINE IN PHARMACEUTICAL FORMULATION BY RP-HPLC ANALYSIS

This study aimed to develop and validate a stability-indicating reverse-phase highperformance liquid chromatography (RP-HPLC) method for the quantification of Rilpivirine in its formulations.The analysis was performed using an HPLC system (Waters -Alliance 510) equipped with UV-484 detector and controlled by Data Ace software (Instrument I.D: AL-011).A hypersil BDS C18 analytical column (250 X 4.6 mm X 5) was employed.A novel mobile phase composed of buffer and acetonitrile in a 20:80 (V/V) ratio was utilized at a flow rate of 1.2 ml/min.Detection was conducted at 300 nm.The stability-indicating nature of the method was evaluated through stress testing, including hydrolytic degradation under acidic, basic, and neutral conditions, UV degradation, and thermal degradation.The method exhibited a linear relationship over the