Integrated analysis of metabolites and enzyme activities reveals the plasticity of central carbon metabolism in grape (<i>Vitis vinifera</i> cv. Cabernet Sauvignon) berries under carbon limitation

High temperatures increase the sugar concentration of grape (<i>Vitis vinifera</i> L.) berries, which can negatively affect the composition and quality of wine, and global climate change is expected to exacerbate this problem. Modifying the source-to-sink ratio of grapevines by selective pruning is a potential strategy to mitigate this. To investigate the effects of low source-to-sink ratio (retaining three leaves per cluster) on carbon metabolism of grape (cv. Cabernet Sauvignon) berries, we conducted an analysis of 42 metabolites and 21 enzyme activities at nine berry developmental stages，as well as transcriptomes from berries grown under two leaves per cluster. The results revealed that the metabolic pathways were coordinately regulated to maintain homeostasis under low source-to-sink ratio conditions. Because of a delay between metabolites and enzyme activities, the metabolites were loosely correlated with enzyme activities, and a lower density of connectivity between them appeared in low source-to-sink conditions. Otherwise, transcripts of the carbohydrate and amino acid metabolism pathways were enriched by carbon limitation. In summary, this integrated analysis reveals a coordinated regulation of various metabolic pathways that maintains the balance of carbon metabolism and ensures survival in challenging environments, highlighting the high metabolic plasticity of grape berries.

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Publication Details

Journal
Horticulture Research
Published
2024-12-28
DOI
https://doi.org/10.1093/hr/uhae363
Primary Topic
Horticultural and Viticultural Research
Type
article
Field-Weighted Citation Impact
0.00

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article

Integrated analysis of metabolites and enzyme activities reveals the plasticity of central carbon metabolism in grape (Vitis vinifera cv. Cabernet Sauvignon) berries under carbon limitation

John E. Lunn, Qian Tong, Bertrand Beauvoit, Zhanwu Dai et al.
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Horticulture Research
Horticultural and Viticultural Research
article

Integrated analysis of metabolites and enzyme activities reveals the plasticity of central carbon metabolism in grape (Vitis vinifera cv. Cabernet Sauvignon) berries under carbon limitation

John E. Lunn, Qian Tong, Bertrand Beauvoit, Zhanwu Dai, Serge Delrot, Regina Feil, Éric Gomès, Yves Gibon, Yi Wang, Yongjian Wang, Xiaobo Xu, Jinliang Chen, Zhenchang Liang, Junhua Kong, Ghislaine Hilbert
article en

Abstract

High temperatures increase the sugar concentration of grape (<i>Vitis vinifera</i> L.) berries, which can negatively affect the composition and quality of wine, and global climate change is expected to exacerbate this problem. Modifying the source-to-sink ratio of grapevines by selective pruning is a potential strategy to mitigate this. To investigate the effects of low source-to-sink ratio (retaining three leaves per cluster) on carbon metabolism of grape (cv. Cabernet Sauvignon) berries, we conducted an analysis of 42 metabolites and 21 enzyme activities at nine berry developmental stages，as well as transcriptomes from berries grown under two leaves per cluster. The results revealed that the metabolic pathways were coordinately regulated to maintain homeostasis under low source-to-sink ratio conditions. Because of a delay between metabolites and enzyme activities, the metabolites were loosely correlated with enzyme activities, and a lower density of connectivity between them appeared in low source-to-sink conditions. Otherwise, transcripts of the carbohydrate and amino acid metabolism pathways were enriched by carbon limitation. In summary, this integrated analysis reveals a coordinated regulation of various metabolic pathways that maintains the balance of carbon metabolism and ensures survival in challenging environments, highlighting the high metabolic plasticity of grape berries.

Horticulture ResearchVol. 12(4)
Université de Bordeaux (FR), Chinese Academy of Sciences (CN), Changzhi Medical College (CN), Beijing Botanical Garden (CN), Ecophysiologie et Génomique Fonctionnelle de la Vigne (FR), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), Biologie du Fruit et Pathologie (FR), École Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (FR), Max Planck Institute of Molecular Plant Physiology (DE), State Key Laboratory of Plant Genomics (CN), Czech Academy of Sciences, Institute of Botany (CZ), Unité mixte de recherche Œnologie (FR), Institute of Botany (CN), University of Chinese Academy of Sciences (CN), China National Botanical Garden, China Agricultural University (CN)
Agence Nationale de la Recherche, National Natural Science Foundation of China, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Climate action
Openalex Percentile: Top 86%
Horticultural and Viticultural Research
DOIOpen Access PDFOpenAlex
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