DEVELOPMENT AND VALIDATION OF ABSORBANCE CORRECTION METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESILATE, OLMESARTAN MEDOXOMIL AND HYDROCHLOROTHIAZIDE

In current study, UV spectroscopy was performed for estimation of Amlodipine Besilate (AMLO), Olmesartan Medoxomil (OLME) and Hydrochlorothiazide (HCTZ).Simultaneous spectrophotometric determination of Amlodipine Besilate, Olmesartan Medoxomil and Hydrochlorothiazide was carried out by absorbance correction method using UV visible double beam spectrophotometer.Validation of simultaneous spectrophotometric method was performed with different parameter like accuracy, precision, recovery, LOD and LOQ.For absorbance correction method, 258 nm, 315 nm and 356 nm were selected as working wavelength because at 315 nm and 258 nm Hydrochlorothiazide, at 258 nm Olmesartan Medoxomil and at 258 nm, 315 nm 356 nm Amlodipine Besilate showed significant absorbance.Linearity of Amlodipine Besilate, Olmesartan Medoxomil and Hydrochlorothiazide was found to be 1-6 g/mL, 2-24 g/mL and 2.5-15 g/mL respectively.The % mean, standard deviation (S.D.), relative standard deviation (% R.S.D.) and standard error (S.E.) were calculated.The % R.S.D. was less than 2% as required by USP and ICH guideline.The experimental work involved is very simple, it requires only measurement of absorbance at selected wavelength.Current study revealed development and validation of